Kroos played for 90 minutes in the Real Madrid's win 3-1 over Paris Saint Germain in the first leg of UEFA Champions League round of 16 on Wednesday, but missed Friday's training session, reported Efe.
"Following tests carried out on Toni Kroos by the Sanitas Real Madrid Medical Services, he has been diagnosed with a sprain to the exterior lateral ligament in the left knee. His recovery will continue to be assessed," the club said in a statement.
Croatian Mateo Kovacic is expected to replace Kroos in Sunday's La Liga match against Real Betis.
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